Table of Contents
What is BCR-ABL quantitative?
The quantitative BCR-ABL1 molecular test measures either of the breakpoints in the fusion gene. It is used to establish a baseline value and then to monitor the person’s response to treatment and, if the person achieves remission, to monitor for recurrence.
What does a positive BCR-ABL mean?
What is it used for? A BCR-ABL test is most often used to diagnose or rule out chronic myeloid leukemia (CML) or a specific form of acute lymphoblastic leukemia (ALL) called Ph-positive ALL. Ph-positive means a Philadelphia chromosome was found. The test is not used to diagnose other types of leukemia.
What is chronic myeloid leukemia BCR-ABL positive?
Definition. Chronic myeloid leukemia (CML), BCR-ABL1-positive, is a myeloproliferative neoplasm (MPN) in which granulocytes are the major proliferative component. It arises in a hematopoietic stem cell and is characterized by the chromosomal translocation t(9;22)(q34.
What was the result of inhibiting BCR-ABL?
Bcr-Abl mutation Point mutations can cause amino acid substitutions inside the kinase domain of the Bcr-Abl protein and disrupt the binding site of imatinib on the tyrosine kinase, resulting in a loss of sensitivity to the drug.
Where does NUP98-HOXA9 occur in the body?
NUP98-HOXA9, the chimeric protein resulting from the t (7;11) (p15;p15) chromosomal translocation, is a prototype of several NUP98 fusions that occur in myelodysplastic syndromes and acute myeloid leukemia. We examined its effect on differentiation, proliferation, and gene expression in primary human CD34 + hematopoietic cells.
Which is the target of the NUP98 gene?
The nucleoporin NUP98 gene is the target of numerous chromosomal rearrangements, predominantly in AML and MDS, resulting in fusion to different partner genes, many of which belong to the homeobox family ( 2 ).
Who is the creator of NUP98 HOXA9 retrovirus?
PG13 cells producing MSCV-IRES-GFP/NUP98-HOXA9 retrovirus were kindly provided by Dr. Malcolm Moore (Sloan-Kettering Cancer Institute, New York, NY). Culture and retroviral transduction of CD34+ primary cells.
How does NUP98-HOXA9 affect erythroid differentiation?
Colony-forming cell (CFC) assays in semisolid medium combined with morphologic examination and flow cytometric immunophenotyping revealed that NUP98-HOXA9 increased the numbers of erythroid precursors and impaired both myeloid and erythroid differentiation.