What is trolox solution?
Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) is a water-soluble analog of vitamin E sold by Hoffman-LaRoche. It is an antioxidant like vitamin E and it is used in biological or biochemical applications to reduce oxidative stress or damage.
How do you calculate trolox equivalent?
2.4 Calculation of Trolox equivalent antioxidant capacity TEAC was calculated as follows: TEAC = IC 50 of Trolox ( μ g / l ) / IC 50 of sample ( μ g / l ) . The higher TEAC value means the higher DPPH radical scavenging activity.
How do you store trolox solution?
For long term storage, we suggest that trolox be stored as supplied at -20°C. It should be stable for at least two years. Trolox is supplied as a crystalline solid. A stock solution may be made by dissolving the trolox in an organic solvent purged with an inert gas.
How do you prepare ABTS stock solution?
The ABTS solution was prepared by mixing an equal volume of a 7 mmol/L ABTS stock solution with a 2.45 mmol/L potassium persulfate solution. The mixture was then stored in the dark at room temperature for 12–16 h.
What is the function of trolox?
Trolox is a water-soluble vitamin E analog with a high capacity to capture ROS. It is used as a standard to check the antioxidant capacity of other molecules. By decreasing oxidant insults to the lung, trolox could modulate the development of chronic lung diseases and lung function decrement.
How do I make trolox standard solution?
Trolox Standard—Standard 6-hydroxy-2,5,7,8-tetrame- thylchroman-2-carboxylic acid (Trolox, 2 mM) should be made by adding 0.05 g of the compound to 100 mL ethanol (75%). It is preferable to adjust the final volume in a volumetric flask. This solution has to be prepared fresh each time.
Is trolox soluble in water?
Trolox | Antioxidant Soluble in DMSO (up to 25 mg/ml) or in Ethanol (up to 25 mg/ml). Also soluble in Water at alkalinepH.
What Colour is ABTS?
In this assay, ABTS is converted to its radical cation by addition of sodium persulfate. This radical cation is blue in color and absorbs light at 415, 645, 734 and 815 nm. The ABTS radical cation is reactive towards most antioxidants including phenolics, thiols and Vitamin C.
Why Trolox is used as standard?
In this method, the Trolox (6-hydroxy-2, 5, 7, 8-tetramethylchroman-2-carboxylic acid) reagent is used as the standard to measure the antioxidant capacity. The assay is based on the reaction of an aqueous solution of ABTS with potassium persulfate solution in the dark for 12–16 h.
How do I make Trolox standard solution?
What does Dpph stand for?
DPPH is a common abbreviation for the organic chemical compound 2,2-diphenyl-1-picrylhydrazyl. It is a dark-colored crystalline powder composed of stable free-radical molecules.
What’s the best way to prepare Trolox stock solution?
1. Prepare 1.5 mMTrolox stock solution, weight 0.0038 gTrolox into 10 volumetric flask and fill uptomark with 90% methanol and store in freezer (at -20oC for up to 2 days) or at -80oC for more than 6 months. 2. Trolox standards (final concentration 0-1.44 mM) depending on the concentration of the sample) in methanol.
What is the solubility of Trolox in water?
The solubility of Trolox at pH 7.2 is 3 mg/mL (12 mM), which is high enough for antioxidant assays.The ABTS antioxidant assay should be used under physiological conditions (100% water, pH 7.4).
What happens when Trolox is used as an antioxidant?
In the presence of Trolox (or of another hydrogen donating antioxidant), the nitrogen atom quenched the hydrogen atom, yielding the solution decolorization, and the absorbance at 743 nm was decreased (Marc, Davin, Deglène-Benbrahim, Ferrand, & Baccaunaud, 2004 ).
Is it safe to put Trolox in the fridge?
Trolox is not light sensitive, do not raise pH > 8, keep a stock solution in a fridge. Trolox does not absorb light in a vis range.