How does QuEChERS method work?
QuEChERS can primarily be thought of as a two-step process: The first step is Extraction, where analytes of interest are extracted from your sample. And the second step is Cleanup, where many potential interferences are removed from the extract.
What is PSA in QuEChERS method?
Primary and secondary amine exchange material (PSA) is the base sorbent used for dSPE cleanup of QuEChERS fruit and vegetable extracts because it removes many organic acids and sugars that might act as instrumental interferences.
Why is acetonitrile used in QuEChERS?
As shown in Table 1, acetonitrile has been the solvent of choice for QuEChERS extractions because it can be easily separated from water by salt partitioning, tends to extract less interfering substances compared with other solvents, and the produced extract can be analyzed by gas or liquid chromatography [2].
What is dispersive SPE?
Dispersive solid phase extraction (d-SPE) analysis is a simple and straightforward sample preparation technique suitable for a wide variety of food and agricultural products. Compared to traditional sample preparation techniques, DisQuE solutions offer: Decreased time spent on sample preparation.
What is sample cleanup?
Protein sample clean up refers to the process of removing non-protein agents that can interfere with the results of downstream processes such as isoelectric focusing (IEF) and 2D electrophoresis.
Are pesticides?
Pesticides are chemical compounds that are used to kill pests, including insects, rodents, fungi and unwanted plants (weeds). Over 1000 different pesticides are used around the world. Pesticides are used in public health to kill vectors of disease, such as mosquitoes, and in agriculture to kill pests that damage crops.
Which law is used in solvent extraction?
THE thermodynamic law for the partition of a solute between two immiscible or partially miscible solvents is: a = K a′ where a and a′ are the activities in the two phases, and K is a constant.
What are the steps in the QuEChERS process?
QuEChERS can primarily be thought of as a two-step process: The first step is Extraction, where analytes of interest are extracted from your sample. And the second step is Cleanup, where many potential interferences are removed from the extract.
How to optimize the extraction salt in QuEChERS?
– Original, Non-buffered: 4 g MgSO4, 1 g NaCl – AOAC: 6 g MgSO4,1.5 g NaAc – EN: 4 g MgSO4, 1 g NaCl, 1 g NaCitrate, 0.5 g disodium citrate sesquihydrate 15 Use one dSPE mixture and keep this part the same for the extraction salt optimization TIP! QuEChERS Extraction Optimization Summary
How long does it take to shake a QuEChERS sample?
Reduces shaking time from 1 minute to <20 seconds! • Consistent extraction of the sample with the salts • Breaks up salt agglomerates • Facilitates homogenization with angle cut • Increase recovery/reduce RSDs of pesticides from sample • 3 different sizes: 50 mL extraction tube and 2 dispersive tubes 15 and 2 mL 11/23/2010
How to use QuEChERS in DSPE Mix 1?
QuEChERS Extraction Optimization Summary dSPE Mix 1 EN Extraction Salts 5982-5650CH Original 5982-5550CH AOAC 2007.01 5982-5755CH 16 Select Representative Sample Homogenize Weigh Mix with salts, solvent Centrifuge