What does a recognition enzyme do?
The restriction enzyme prevents replication of the phage DNA by cutting it into many pieces. These regions are called recognition sequences, or recognition sites, and are randomly distributed throughout the DNA. Different bacterial species make restriction enzymes that recognize different nucleotide sequences.
What type of enzyme is HindIII?
HindIII (pronounced “Hin D Three”) is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the DNA palindromic sequence AAGCTT in the presence of the cofactor Mg2+ via hydrolysis.
What is enzyme BamHI?
BamHI (from Bacillus amyloliquefaciens) is a type II restriction endonuclease, having the capacity for recognizing short sequences (6 bp) of DNA and specifically cleaving them at a target site. This allows the DNA to maintain its normal B-DNA conformation without distorting to facilitate enzyme binding.
Where does the HindIII enzyme cut?
The restriction enzyme HindIII cuts DNA at the sequence AAGCTT, and the restriction enzyme HpaII cuts DNA at the sequence CCGG.
What is the rate of the acciii enzyme?
AccIII (BspMII) restriction site: Effect of DNA methylation: This enzyme is capable of cleaving TC 5mCGGA, but its rate is about 1/75. This enzyme is capable of cleaving TC5mCGGA, but its rate is about 1/75.
Which is the best restriction enzyme from Takara?
DNA Restriction Enzymes from Takara such as AccIII are high-quality: perform restriction enzyme digestion with reliable restriction endonucleases. Cat. # 1113B contains 5 of Cat. # 1113A.
Which is E coli strain carries the ACCI gene?
An E. coli strain that carries the AccI gene from Acinetobacter calcoaceticus (R. Roberts). One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
When to use Thermo Fisher Scientific kpn2i restriction enzyme?
Order requests are processed and shipped by your local distributor. Learn more Thermo Scientific Kpn2I (BspEI) restriction enzyme recognizes T^CCGGA sites and cuts best at 55°C in Tango buffer.