Table of Contents
What is spectrum viewer?
The BD Spectrum Viewer is a tool that depicts the excitation and emission curves of fluorochromes common to flow cytometry. This tool can be used to determine appropriate filters to detect a fluorochrome as well as fluorochrome compatibility and fluorescent spillover.
What is BV421?
BD Horizon™ Brilliant Violet™ 421 (BV421) (Exmax 407 nm/Emmax 421 nm) is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. This dye offers improved brightness over Pacific Orange™ and reduced spillover into the FITC channel when compared to AmCyan.
Is af647 the same as APC?
Alexa Fluor 647 (Ex-Max 650 nm/Em-Max 668 nm): conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC, albeit APC tends to be brighter. However, Alexa Fluor 647 is considered more optimal for intracellular applications.
How bright is BV421?
For cell surface staining, BV421™ is significantly brighter than other spectrally equivalent fluorophores including Pacific Blue™, BD Horizon™ V450, eFluor® 450, and Alexa Fluor® 405. It has an extinction coefficient of 2,500,000 M-1cm-1 at 405 nm, and an aqueous solution quantum yield of 65 ± 5%.
How is fluorescence detected?
In some cases, when the light energy is absorbed by a molecule, it raises some of the electrons to an excited state. When these electrons return to the ground state and light is emitted, the process is referred to as fluorescence. Fluorescence detectors rely on this molecular property for detection.
What color is PE fluorescence?
red-orange
R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields.
What can the BD Spectrum Viewer be used for?
The BD Spectrum Viewer is a tool that depicts the excitation and emission curves of fluorochromes common to flow cytometry. This tool can be used to determine appropriate filters to detect a fluorochrome as well as fluorochrome compatibility and fluorescent spillover.
Why do we need a BD healthsight viewer?
BD HealthSight Viewer improves visibility to bottlenecks in pharmacy fulfillment to optimize medication availability and improve visibility between Pharmacy and Nursing. Provide Nursing and Pharmacy with the delivery status of medications, removing blind spots and wasted time and effort.
How does BD-viewer 12.000-softpedia work?
It is able to display PC board information, panel and footprint data without depending on the CD-5000 execution environment. The tool reveals the PC board data, footprint library or panel info with the aid of a tree-like display containing the corresponding values (e.g. components and nets, footprints).
What does BD Biosciences do for a living?
BD Biosciences provides flow cytometers, reagents, tools, and a wide range of services to support the work of researchers and clinicians who understand disease and improve care.
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