What is the purpose of diafiltration?
Diafiltration is a technique that uses ultrafiltration membranes to completely remove, replace, or lower the concentration of salts or solvents from solutions containing proteins, peptides, nucleic acids, and other biomolecules.
Is ultrafiltration and dialysis the same?
Ultrafiltration is the removal of fluid from a patient and is one of the functions of the kidneys that dialysis treatment replaces.
What is a Diavolume?
A diavolume is defined as the “total buffer volume introduced to the operation during diafiltration [divided] by the [initial] retentate volume” [7,8].
What is a TFF?
Tangential flow filtration (TFF), also known as Cross-flow filtration, is a process of separation widely used in bio-pharmaceutical and food industries. This method is preferred for its continuous filtration and reproducible performance.
What is retentate and permeate?
Permeate designates the liquid passing through the membrane, and retentate (concentrate) designates the fraction not passing through the membrane.
Does dialysis remove creatinine?
Dialysis removes fluid and wastes Waste such as nitrogen and creatinine build up in the bloodstream. If you have been diagnosed with CKD, your doctor will have these levels carefully monitored. One of the best indicators of kidney function is your glomerular filtration rate (GFR).
What particles are removed from the blood in dialysis?
Dialysis removes the waste products and extra fluid from your blood by filtering them through a membrane/filter, similar to the way healthy kidneys would. During dialysis, blood is on one side of the membrane/filter and a special fluid called dialysate (containing water, electrolytes, and minerals) is on the other.
What is NWP in TFF?
Key elements demonstrating cleaning effectiveness in a TFF system. Normalized Water. Permeability (NWP) Normalized water permeability (NWP) is an established method for determining the cleanliness of a cassette after cleaning.
What is TMP pressure?
The pressure that is needed to press water through a membrane is called Trans Membrane Pressure (TMP). The TMP is defined as the pressure gradient of the membrane, or the average feed pressure minus the permeate pressure.
What is the difference between dialysis and ultrafiltration?
Dialysis and ultrafiltration are two processes that relate to our kidney function. In both processes, waste materials in our blood filter out through a semi-permeable membrane. Both processes prevent larger molecules to pass through a membrane.
Which is better diafiltration or centrifugal buffer exchange?
Dialysis can require large buffer volumes and multiple buffer changes. In contrast, diafiltration uses ultrafiltration membranes, either in centrifugal or pressure-driven devices such as the Amicon® Stirred Cell, for efficient buffer exchange.
Which is more efficient continuous diafiltration or discontinuous diafiltration?
By maintaining a fixed volume, the retained solute concentration remains constant, providing a gentler method of buffer exchange. In general, continuous diafiltration is more efficient than discontinuous diafiltration.
Can a diafiltration buffer be used with a stirred cell?
While continuous diafiltration is traditionally used with large volume tangential flow filtration (TFF) systems, you can use this gentle form of buffer exchange with the Amicon® Stirred Cell. The same reservoir used to extend the processing volume during sample concentration can now be used for the diafiltration buffer.