What is fab in mass spectrometry?
Fast atom bombardment (FAB) is an ionization technique established in 1981, which is widely used as an ion source in mass spectrometry systems. Unlike other ionization techniques, FAB bombards a sample with a neutral atom (Xe, Ar) to ionize the sample.
What is Fab Spectroscopy?
Fast atom bombardment (FAB) is an ionization technique used in mass spectrometry in which a beam of high energy atoms strikes a surface to create ions. When a beam of high energy ions is used instead of atoms (as in secondary ion mass spectrometry), the method is known as liquid secondary ion mass spectrometry (LSIMS).
Which gas is used in FAB MS for ionisation?
Fast-atom bombardment (FAB-MS) uses a beam of neutral atoms (argon or xenon) bearing a high kinetic energy for the ionization of involatile ionic or nonionic compounds present either in the solid state or as a solution in a glycerol matrix.
How many mg sample is required for FAB method?
We require 0.5 mg of sample for FAB and direct probe EI /CI analyses (though we can often make do with less when only a small amount of sample exists – please seek advice PRIOR to submission).
How does Maldi-TOF MS work?
In the case of MALDI-TOF, the analyzer separates molecules based on the time it takes each of them to fly through the time-of-flight tube or “drift” region to the detector. The ionized sample molecules are accelerated by a high-voltage current and fly through the tube before striking the detector.
What is TOF in Maldi-Tof?
In time of flight (TOF) mass spectrometry, ionized oligo molecules are accelerated by an electrostatic field in the mass analyzer to a common kinetic energy. With the same kinetic energy, lighter ions travel faster and heavier ions travel more slowly.
How does MALDI-TOF MS work?
What does MALDI-TOF MS do?
The MALDI-TOF can be used in profiling and imaging proteins directly from thin tissue sections, known as MALDI imaging mass spectrometry (MALDI-IMS). It provides specific information about the local molecular composition, relative abundance and spatial distribution of peptides and proteins in the analyzed section.
What is the principle of MALDI-TOF?
MALDI is the abbreviation for “Matrix Assisted Laser Desorption/Ionization.” The sample for MALDI is uniformly mixed in a large quantity of matrix. The matrix absorbs the ultraviolet light (nitrogen laser light, wavelength 337 nm) and converts it to heat energy.