Table of Contents
How long does GFP fluorescence last?
The half-life of the green fluorescent protein (GFP) was determined biochemically in cultured mouse LA-9 cells. The wild-type protein was found to be stable with a half-life of ~26 h, but could be destabilized by the addition of putative proteolytic signal sequences derived from proteins with shorter half-lives.
What is Venus fluorescent protein?
Venus is a basic (constitutively fluorescent) yellow fluorescent protein published in 2002, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with moderate acid sensitivity.
Can you fix cells with GFP?
There really is no need for fixing them; just image the live cells. If you want a nuclear stain in addition to the GFP signal, you can use Draq5, which is cell permeable and will give you the same information as DAPI. The only reason for fixing cells to detect GFP is if you also need to stain intracellular antigens.
Where does red fluorescent protein come from?
The Fluorescent Protein Color Palette Longer wavelength fluorescent proteins, emitting in the orange and red spectral regions, have been developed from the marine anemone, Discosoma striata, and reef corals belonging to the class Anthozoa.
Can you use GFP and YFP together?
GFP and YFP signals can be discriminated using a customized filter configuration involving a 550/30-nm band pass for YFP, a 510/20-nm band pass filter for GFP, with a 525-nm short-pass dichroic mirror between them (2).
What kind of fluorescent protein is the Venus?
Venus is a basic (constitutively fluorescent) yellow fluorescent protein published in 2002, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with moderate acid sensitivity. compare Comparison List Add Venus show comparison clear selection FPbase info
What kind of protein is a yellow fluorescent protein?
Venus is a basic (constitutively fluorescent) yellow fluorescent protein published in 2002, derived from Aequorea victoria.
How to obtain yellow fluorescent protein ( YFP ) with restriction sites?
Yellow fluorescent protein (YFP) with fast and efficient maturation. To obtain this DNA and protein sequence with restriction sites, please download SnapGene or the free SnapGene Viewer. Your time is valuable! × Please choose an application for opening sequence files.
How are fluorescent proteins expressed in a fibroblast?
D. Co-expression of an endoplasmic reticulum targeted red fluorescent protein and a Golgi complex targeted GFP in a mammalian fibroblast. Scale bars = 10μm. Fluorescent Protein Expression Plasmid Features When purchasing or requesting an FP plasmid, you will often be asked to select the “N” or “C” version.