Where do VERO cells come from?
Vero cells are derived from the kidney of an African green monkey, and are one of the more commonly used mammalian continuous cell lines in microbiology, and molecular and cell biology research.
What is HEp-2 cell line?
Hep-2/Hep2 cells are invaluable for researchers engaged in the analysis of autoantibodies, and they are currently one of the most common substrates for antinuclear antibody detection by immunofluorescence [6,7]. The Hep-2 cell line was first specifically described by H.W. Toolan in 1954 .
Where do immortal cell lines come from?
Immortalized cell lines are derived from a variety of sources that have chromosomal abnormalities or mutations that permit them to continually divide, such as tumors. Because immortalized cells continuously divide, they eventually fill up the dish or flask in which they are growing.
Where do HEp-2 cells come from?
The HEp-2 cell line originated from tumours produced after injection of rats with epidermoid carcinoma tissue.
Why are Vero cells used for viruses?
Firstly, the cell bank is easy to establish and preserve, at the same time it can be continuously passaged with a fast growth rate. Secondly, Vero cells have stable genetic traits and a low probability of malignancy. Thirdly, Vero cells are sensitive to a variety of viruses and have high virus titers.
Are HeLa cells cancerous?
1- HeLa cells are cancerous. The difference between normal cells and HeLa cells is most visible when you look at the chromosomes (karyotype).
Are all cell lines cancerous?
There are various immortal cell lines. Some of them are normal cell lines (e.g. derived from stem cells). Other immortalised cell lines are the in vitro equivalent of cancerous cells.
Are Vero cells immortal?
Since they are derived from normal kidney cells and not immortal cells like HeLa, Vero cells retain the attributes of normal cells, notably cell contact inhibition.
Why HeLa cells are so important?
HeLa cells have been used to test the effects of radiation, cosmetics, toxins, and other chemicals on human cells. They have been instrumental in gene mapping and studying human diseases, especially cancer. HeLa cells were used to maintain a culture of polio virus in human cells.
What kind of cell line is Hep G2?
Hep G2 Cells. Hep G2 (or HepG2) is a human liver cancer cell line. Hep G2 is an immortal cell line which was derived from the liver tissue of a 15-year-old American adolescent boy of European ancestry with a well-differentiated hepatocellular carcinoma.
How are HepG2 cells used in immunocompromised mice?
HepG2 cells are inoculated in immunocompromised mice to create the HepG2 Cell Line Derived Xenograft (CDX) mouse model. The HepG2 xenograft of human hepatocellular carcinoma (HCC) enables studies targeting antiangiogenesis (i.e. rapamycin, bevacizumab) or tumor growth inhibition (e.g. sorafenib).
How are HepG2 cells used in drug metabolism?
The HepG2 cell line is commonly used in drug metabolism and hepatoxicity studies. HepG2 cells exhibit an epithelial-like morphology with a modal chormosome number of 55.
Which is hepatocellular carcinoma cell line is immortalized?
HepG2 is an immortalized cell line consisting of human liver carcinoma cells, derived from the liver tissue of a 15-year-old Caucasian male who had a well-differentiated hepatocellular carcinoma, which is the fifth most-common cancer worldwide. The HepG2 cell line is commonly used in drug metabolism and hepatoxicity studies.