How alginate lyase is used for treatment of cystic fibrosis?
Alginate lyase treatment has been shown to reduce viscosity both in cultures of clinical isolates and in CF sputum29; it strips biofilms from abiotic surfaces22,44, enhances phagocytosis of P. aeruginosa by human immune cells20,23,45, and improves the efficacy of various antipseudomonal antibiotics18,19,45,46.
Why would DNase I and alginate lyase be useful for treating cystic fibrosis?
Recent publications have focused on the treatment of CF patients with recombinant human DNase (rhDNase), which cleaves DNA and decreases sputum viscosity, improves FEV and reduces the risk of pulmonary exacerbations.
How do you dissolve alginate lyase?
To dissolve alginate gels, usually a (55mM, pH=6.8) sodium citrate solution is used.
How do biofilms cause cystic fibrosis?
The chronic inflammation is the major cause of the lung tissue damage in CF. Biofilm growth in CF lungs is associated with an increased frequency of mutations, slow growth and adaptation of the bacteria to the conditions in the lungs, and to antibiotic therapy.
Can alginate be digested?
In summary, the sodium alginate-calcium carbonate system is capable of forming a gel inside the stomach and encapsulating nutrients in the gel matrix. During the digestion process, the digestive enzymes in the gastric digestion stage have only as light effect on the shape and nutrient release of the gel.
How does alginate lyase work?
Alginate lyase causes detachment of a biofilm by reducing its adhesion to the surfaces, and increases phagocytosis and antibiotic susceptibility. In this study, alginate lyase was purified in just one step and its properties were investigated.
How is biofilm developed on the surface?
Biofilm formation is a process whereby microorganisms irreversibly attach to and grow on a surface and produce extracellular polymers that facilitate attachment and matrix formation, resulting in an alteration in the phenotype of the organisms with respect to growth rate and gene transcription.
Is alginate made from seaweed?
The raw material of Alginate is brown seaweed which grows abundantly in the world’s oceans. KIMICA utilizes Lessonia as its raw material seaweed.