What is o-dianisidine dihydrochloride?
o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm.
What does O-Dianisidine do?
Product Description This product is a preweighed vial containing 50 mg of o-dianisidine dihydrochloride, which is a peroxidase substrate used in the quantitative determination of glucose in aqueous solutions such as serum, using a coupled enzyme system with glucose oxidase and peroxidase (PGO enzymes).
Is Dianisidine dihydrochloride a dye?
Benzidine derivatives, such as 3,30-dimethylbenzidine and 3,30-dimethoxybenzidine (o-dianisidine) (Figure 25), are used as dyes or intermediates for dyestuffs or pigments (e.g., Trypan Blue, Acid Red 14, Direct Blue 1, 8, 15, 76, 98, 218, and Pigment Orange 16), coatings, plastics, or in chemical studies.
What does O Dianisidine enzyme reagent test for?
The o-dianisidine assay measures the rate of change in the amplitude of the 449 nm absorption peak and is sensitive for solutions containing enzyme concentrations of up to 3 U/ml. 17-20 This assay is useful in the study of immobilized enzyme, where dilutions are not possible.
Which is the correct formula for o dianisidine?
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). o-Dianisidine is an organic compound with the formula [ (CH 3 O) (H 2 N)C 6 H 3] 2. A colorless or white solid, it is a bifunctional compound derived via the benzidine rearrangement from o -anisidine .
Can a direct blue dye be o dianisidine?
Where the ROOR’ can be hydrogen peroxide, and the electron doner be o -dianisidine. Direct Blue 1 is commercial dye, a derivative of o-dianisidine. The manufacture and degradation of o -dianisidine, like other benzidene derivatives, has attracted regulatory attention.
Which is the precursor to azo dye o-dianisidine?
o -Dianisidine is a precursor to some azo dyes by formation of the bis( diazonium) derivative, which is coupled to diverse aromatic compounds. Some commercial dyes derived from o -dianisidine include C. I. Direct Blue 1, 15, 22, 84, and 98. o -Dianisidine is also used in assaying activity of peroxidase in lab.
What’s the difference between chlorhydrate and hydrochloride?
In chemistry, a hydrochloride is an acid salt resulting, or regarded as resulting, from the reaction of hydrochloric acid with an organic base (e.g. an amine). An alternative, more elegant name is chlorhydrate, which comes from French. An archaic alternative name is muriate, derived from hydrochloric acid’s ancient name: muriatic acid.