Is guaiacol an inhibitor?
Guaiacol is a competitive inhibitor of purified GYS1 and a mixed inhibitor of the enzyme in cell lysate.
Is peroxidase an inhibitor?
The inhibition kinetics demonstrates a noncompetitive effect. In conclusion, the inositol phosphoglycan-like compound is the general inhibitor of peroxidases and catalase involved in the modulation of hydrogen peroxide level that acts in different metabolic pathways as a signal transducer.
What is the role of guaiacol?
The compound guaiacol has a high affinity for oxygen, and in solution, it binds instantly with oxygen to form tetraguaiacol, which is brownish in color. The greater the amount of oxygen produced, the darker brown the solution will become.
Is guaiacol a substrate or product?
Determination of enzymatic characteristic properties such as Michaelis–Menten constant (Km), temperature optima and pH optima using different phenolic compounds, indicated p-cresol as a potential substrate for the peroxidase enzyme assay at room temperature, whereas guaiacol, which is widely used as a substrate for …
Where is guaiacol found?
Although it is biosynthesized by a variety of organisms, this aromatic oil is usually derived from guaiacum or wood creosote. It is also found in essential oils from celery seeds, tobacco leaves, orange leaves, and lemon peels.
What are the inhibitors of catalase?
IT is well known that the activity of catalase is greatly inhibited by very small concentrations of potassium cyanide, hydrogen sulphide and especially hydroxylamine.
How is guaiacol formed?
Guaiacol is produced in the gut of desert locusts, Schistocerca gregaria, by the breakdown of plant material. This process is undertaken by the gut bacterium Pantoea agglomerans (Enterobacter). It is one of the main components of the pheromones that cause locust swarming.
Is guaiacol soluble in water?
One gram of guaiacol dissolves in 60-70 ml of water or 1 g of glycerol. It is miscible with alcohol, chloroform, ether, oils, glacial acetic acid, and slightly soluble in petrolium ether.
Is guaiacol toxic?
Guaiacol is a compound used as expectorant. We concluded that guaiacol is an extremely toxic product (toxic rating class 5) whose use should be restricted or banned.
How are oxidation rates of guaiacol inhibited by azide?
Guaiacol or o-dianisidine oxidation rates were increased by CaCl2and inhibited by potassium cyanide and azide. When proteins solubilized by SDS from non-washed PM were separated by SDS-PAGE, two bands (38 and 45 kD) could be detected by hemestaining.
What kind of agent is guaiacol used for?
Guaiacol is an agent thought to have disinfectant properties and used as an expectorant. (From Martindale, The Extra Pharmacopoeia, 30th ed, p747) Guaiacol is a phenolic compound with a methoxy group and is the monomethyl ether of catechol.
Which is the oxidized co substrate of guaiacol?
Guaiacol. Guaiacol is readily oxidized by the heme iron of peroxidases including the peroxidase of cyclooxygenase (COX) enzymes. It therefore serves as a reducing co-substrate for COX reactions. Guaiacol is a phenolic natural product first isolated from Guaiac resin and the oxidation of lignin.
What does guaiacol stand for in medical category?
Guaiacol. Guaiacol; 2-Methoxyphenol; 90-05-1; O-Methoxyphenol; 2-Hydroxyanisole; Phenol, 2-methoxy- More… Guaiacol is an agent thought to have disinfectant properties and used as an expectorant. Guaiacol is a phenolic compound with a methoxy group and is the monomethyl ether of catechol.