## What does a 100 fold dilution mean?

For a 1:100 dilution, one part of the solution is mixed with 99 parts new solvent. Mixing 100 µL of a stock solution with 900 µL of water makes a 1:10 dilution. The final volume of the diluted sample is 1000 µL (1 mL), and the concentration is 1/10 that of the original solution.

### How do you do a 100X dilution?

a) To get from 100X to 1X, the dilution factor is 100 (think of it was what do you divide 100 by to get to 1). So once you know this, divide 1 M by 100, to give you 0.01M or 10 mM.

**What is a 200 fold dilution?**

200 (1/200 dilution) = the first step in the dilution factor; the second is 1/50, obtained as follows: 1 ml of serum + 199 ml of diluent = 1/200 dilution. 1 ml of 1/200 dilution + 49 ml of diluent = 1/50.

**What is a 10 fold dilution?**

A ten-fold dilution reduces the concentration of a solution or a suspension of virus by a factor of ten that is to one-tenth the original concentration. A series of ten-fold dilutions is described as ten-fold serial dilutions.

## What is a 4 fold dilution?

For example, creating a four-fold dilution (DF = 1/4) with a starting concentration of 10 µM yields the following concentrations: 10 µM, 2.5 µM, 0.625 µM, 0.156 µM, 0.039 µM etc. Video 1. Serial dilution tutorial. For assistance preparing a serial dilution, see ‘Serial Dilution Calculator.

### What does a dilution factor of 2 mean?

For example, if 100 mL of a stock solution is diluted with solvent/diluent to a total, final volume of 1000 mL, the resulting dilution factor is 10. For this particular dilution, it may also be said that the stock solution was diluted 10-fold. As another example, a 2-fold dilution is the same as a dilution factor of 2.

**How is dilution factor calculated?**

Dilution factor is defined as: total volume of solution per aliquot volume. Where total volume of solution is: 10.0 + 240.0 = 250.0 mL (volumetric flask.) Note: For multiple dilutions the dilution factor is the product of the dilution factors for each individual dilution.

**What does 10-fold mean?**

1 : being 10 times as great or as many. 2 : having 10 units or members. Other Words from tenfold Example Sentences Learn More About tenfold.

## What is a 5 fold dilution?

“It was a 5 fold dilution” “It was diluted 1/5″ These all mean the same thing, that there is 1 volume part of sample and 4 volume parts of whatever liquid is being used to dilute the sample for a total of 5 volume parts.

### How do you do a 20 fold dilution?

For example, a 1:20 dilution converts to a 1/20 dilution factor. Multiply the final desired volume by the dilution factor to determine the needed volume of the stock solution. In our example, 30 mL x 1 ÷ 20 = 1.5 mL of stock solution.