How much DEPC do you add to water?
- Add 1ml of 0.1% Diethylpyrocarbonate (DEPC) to 1000ml distilled water.
- Mix well and let set at room temperature for 1 hour.
- Let cool to room temperature prior to use.
How do you make DEPC-treated water?
Add 1 ml DEPC (Diethylpyrocarbonate) per 1000 ml of MilliQ or double distilled water (i.e. to a final concentration 0.1%) and mix thoroughly. 3. Let the DEPC-mixed water incubate for 12 hours at 37°C.
What is the pH of DEPC-treated water?
|Grade:||Endonuclease-Free, Exonuclease-Free, Molecular Biology Grade, RNase-Free, DNase-Free|
Does DEPC-treated water expire?
DEPC-Treated Water is shipped on dry/blue ice. On arrival store at -20°C for optimum stability. When stored under the recommended conditions and handled correctly, full activity is retained until the expiry date on the outer box label.
Does DEPC treated water expire?
What is the pH of RNase free water?
between 5.0 and 6.5
What is the pH value of QIAGEN’s Nuclease-Free Water? At 22°C, Nuclease-Free Water has a pH value of between 5.0 and 6.5.
Is DEPC water Toxic?
1. DEPC is toxic and not appropriate in a lab where it cannot be handled properly. This objection is with merit, as improper handling may present a serious health risk. DEPC is too difficult to remove from reagents and interferes with PCR and other re-actions.
What should be the concentration of DEPC in water?
Abstract: Solutions (water and other solutions) should be treated with 0.1% DEPC. DEPC is a strong, but not absolute, inhibitor of RNases. It is commonly used at a concentration of 0.1% to inactivate RNases on glass or plasticware or to create RNase-free solutions and water. DEPC inactivates RNases by covalent modification.
How long does it take to inactivate DEPC in water?
Water is usually treated with 0.1% v/v DEPC for at least 2 hours at 37 °C and then autoclaved (at least 15 min) to inactivate traces of DEPC. Inactivation of DEPC in this manner yields CO 2 and ethanol. Higher concentrations of DEPC are capable of deactivating larger amounts of RNase, but remaining traces…
When to use DEPC or RNase free water?
DEPC-treated (and therefore RNase-free) water is used in handling of RNA in the laboratory to reduce the risk of RNA being degraded by RNases. Water is usually treated with 0.1% v/v DEPC for at least 2 hours at 37 °C and then autoclaved (at least 15 min) to inactivate traces of DEPC.
How is DEPC treated water used in molecular biology?
DEPC-Treated Water Features Clear, Liquid Solution DNase & RNase-Free Commonly used in molecular biology and biochemistry applications Free of impurities Relatively long-storage when handled and stored properly under defined conditions