What is the purpose of adding resazurin dye to the fluid thioglycollate broth?
Resazurin is an oxidation-reduction indicator that turns pink when increased oxidation has occurred, it is colorless when reduced. Agar: The addition of a small amount of agar in Thioglycollate medium aids in the initiation and growth of small inocula and anaerobes by impeding the diffusion of oxygen into the medium.
How do you inoculate thioglycollate broth?
Bring to the boil and dissolve the medium completely. Distribute into tubes or bottles and sterilize by autoclaving at 121°C for 15 minutes. Inoculate the medium following aseptic technique. Once inoculated, the broth should be incubated at 35-35°C as soon as possible.
What does the pink layer at the top of the thioglycollate media indicate?
The pink layer indicates the area where oxygen is present. When evaluating tubes of FTM (Fluid Thioglycollate Media) it is important not to agitate the tubes because the position of growth in the medium can be easily changed if handled carelessly.
Which bacteria grow at the top of a Thioglycolate tube and which grow at the bottom of the tube?
Aerobic and anaerobic bacteria can be identified by growing them in test tubes of thioglycolate broth:
- Obligate aerobes need oxygen because they cannot ferment or respire anaerobically.
- Obligate anaerobes are poisoned by oxygen, so they gather at the bottom of the tube where the oxygen concentration is lowest.
What is the purpose of the resazurin in a fluid thioglycollate broth tube?
What is the purpose of the resazurin in a fluid thioglycollate broth tube? Resazurin is an oxidation-reduction indicator that turns pink when increased oxidation has occurred, it is colorless when reduced.
Why is thioglycollate broth used in bacteriology?
Thioglycollate broth is the enrichment broth most frequently used in diagnostic bacteriology. This broth supports the growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms. It contains many nutritive factors, including casein, yeast, beef extracts, and vitamins to enhance the growth of the most medically important bacteria.
What is the purpose of BD fluid thioglycollate medium?
BD Fluid Thioglycollate Medium (FTM) INTENDED USE BD Fluid Thioglycollate Medium is a general purpose liquid enrichment medium used in qualitative procedures for the sterility test and for the isolation and cultivation of aerobes, anaerobes and microaerophiles that are not excessively fastidious. In clinical microbiology, it