What does NeuN stain for?
NeuN (neuronal nuclei) antibodies are fundamental tools for staining mature neurons and studying neuronal development and differentiation.
What can be used as a marker for dendrites?
MAP2 for dendrites and Tau for axons are the right markers. You should be careful in NOT using Phosphorylated-Tau antibody as it also marks the dendrites.
What are neural markers?
A neuronal lineage marker is an endogenous tag that is expressed in different cells along neurogenesis and differentiated cells such as neurons. It allows detection and identification of cells by using different techniques. A neuronal lineage marker can be either DNA, mRNA or RNA expressed in a cell of interest.
What cells does NeuN stain?
The mAb staining is localized primarily to nuclei. Thus, the authors named the antigen recognized by this mAb NeuN for “Neuronal Nuclei.” The original study and subse- quent studies by others have shown that anti-NeuN stains most types of neurons throughout the nervous system with few exceptions (1, 2).
What is NeuN a marker of?
Neuronal nuclear antigen (NeuN): a marker of neuronal maturation in early human fetal nervous system. Brain Dev.
What do GABAergic neurons do?
GABAergic neurons produce gamma-Aminobutyric acid (GABA), which is the main inhibitory neurotransmitter in the mammalian central nervous system (CNS). Dysfunction of GABA neurotransmission can result in several disorders, including schizophrenia and epilepsy.
Are GABAergic neurons excitatory?
GABA is the main inhibitory neurotransmitter in the adult brain. Early in development, however, GABAergic synaptic transmission is excitatory and can exert widespread trophic effects.
How do you stain neurons?
Golgi staining is the oldest such method. In Golgi staining, silver nitrate is introduced to fixed tissue, and the metal is taken up by a sub-population of neurons through a mechanism that remains largely uncharacterized. Neurons are stained in their entirety and then imaged with light microscopy (Koyama, 2013).
Are axons or dendrites thicker?
Axons tend to be thinner than dendrites, which is another reason they can be more difficult to track down. If you are unsure whether or not you are looking at an axon, look at the thickness of the other neurites around it to compare. An axon should almost always be thinner.