Table of Contents
What are isoschizomers used for?
Recognition site and cleavage specificity Isoschizomers are restriction enzymes that have the same recognition sequence and the same specificity. For instance, AgeI and BshT1 recognize and cleave 5′-A↓CCGGT-3′ in the same pattern.
What are Isocaudomers explain with suitable examples?
Isocaudomers are restriction endonucleases that produce the same nucleotide extensions but have different recognition sites. The examples are NcoI from Nocardia corallina and PagI from Pseudomonas alcaligenes. They bind different DNA sequences but produce the same sticky ends.
Why Type II enzymes are usually used for cloning?
Type II restriction enzymes are the familiar ones used for everyday molecular biology applications such as gene cloning and DNA fragmentation and analysis. These enzymes cleave DNA at fixed positions with respect to their recognition sequence, creating reproducible fragments and distinct gel electrophoresis patterns.
What is the advantage of sticky ends in cloning versus blunt ends?
Because sticky ends find each other faster due to their attraction for each other, the process of ligation requires less human DNA and less plasmid DNA. The blunt ends of DNA and plasmids are less likely to find each other, and thus ligation of blunt ends requires that more DNA is put into the test tube.
What are the three core values of Neb?
Practicing Responsible Science From placing the advancement of science and the stewardship of the environment as our highest priorities, to our unique corporate culture, NEB’s philosophy can be distilled down to three core values: passion, humility, and being genuine.
Where can I get an isoschizomer for neb?
The list below contains isoschizomers for commercially available restriction endonucleases. It also specifies which isoschizomer is available from New England Biolabs. All recognition sequences are written 5´ to 3´ using the single letter code nomenclature with the point of cleavage indicated by a “/”.
Why is Neb the leader in restriction enzymes?
Having supplied restriction enzymes to the research community for over 45 years, NEB has earned the reputation of being the leader in enzyme technologies. Working continuously to be worthy of that distinction, NEB strives to develop enzyme of the highest purity and unparalleled quality.
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