What is the principle of fluorimetry?
Principle of Fluorimetry: When molecules are irradiated with light of the appropriate frequency, it will be absorbed in about 10-15 seconds. In the process of absorption, the molecules may move from ground to the first excited singlet electronic state.
What is fluorimetry Slideshare?
When a beam of light is incident on certain substances they emit visible light or radiations. This is known as fluorescence. Fluorescence starts immediately after the absorption of light and stops as soon as the incident light is cut off. The substances showing this phenomenon are known as flourescent substances.
Which detector is used in fluorimetry Mcq?
Mainly photovoltaic cell, Photo multiplier tubes , Photo tube are used as detector. Photo multiplier tubes are the best and accurate.
What are the advantages and disadvantages of Fluorometry?
Advantages and Disadvantages As it was already pointed, one of the most important advantages of this technique is due to its high sensitivity and specifity. Another is its fast and rapid diagnosis ability. The main disadvantage is that not all compounds fluoresce.
How is fluorescence used in medicine?
Fluorescence spectroscopy is an emerging diagnostic tool for various medical diseases including pre- malignant and malignant lesions. Fluorescence spectroscopy is a noninvasive technique and has been applied successfully for the diagnosis of multisystem cancers with high sensitivity and specificity.
Which detector is the best choice for fluorimetry?
Why are detectors placed at 90 degrees?
The sample emits a wavelength, which travels to the detector. The detector is usually set at a 90-degree angle to the light source to avoid any interference from the transmitted excitation light. Photons emitted hit a photo detector.
Which is the best slide for fluorimetry presentation?
1. FLUORIMETRY PRESENTED BY V.SREE VIDYA (14AB1S0408) UNDER GUIDENCE OF CH.DEVDAS DEPARTMENT OF PHARMACEUTICAL ANALYSIS 2. CONTENTS Principle Factors effecting fluorescence intensity Instrumentation Applications Conclusion References.
What is the difference between phosphorimetry and fluorimetry?
FLUORIMETRY It is measurement of fluorescence intensity at a particular wavelength with the help of a filter fluorimeter or a spectrofluorimeter. 3. Phosphorimetry A form of fluorimetry in which phosphorescence of a sample is measured in a conjunction with a pulsed sourse of radiation. 4.
How does fluorometry work in a SlideShare app?
FLUOROMETRY BASIC CONCEPTS, INSTRUMENTATION AND APPLICATIONS By Dr. Basil, B – MBBS (Nigeria), Department of Chemical Pathology/Metabolic Medicine, Benue State… Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising.
When does the intensity of fluorimetry become praportnal?
Fluorescence intensity is praportnal to concentration of substance only when the absorbance is less than 0.02 A=log IoIt or A= abc Io=intensity of incident light a= absorptivity of constant b= Pathlength c= concentration Concentration:- 13.